CHO-K1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Thermo Fisher Scientific) supplemented with 5% (v/v) of a 1:1 mix of calf bovine serum and fetal bovine serum and 1% penicillin-streptomycin. Cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. Cells were transfected with LipoD293 DNA Transfection Reagent from SignaGen Laboratories (catalog no. SL100668). The quantity of DNA for each plasmid varied based on the assay; however, the ratio receptor/accessory protein was constant and set at 1:20.

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