Construction of manA mutant
This protocol is extracted from research article:
Slow growth determines nonheritable antibiotic resistance in Salmonella enterica
Sci Signal, Jul 30, 2019; DOI: 10.1126/scisignal.aax3938

A ΔmanA::cat mutation was generated by recombineering using a purified linear DNA fragment generated from a PCR reaction using primers 12460 and 12461 and plasmid pDK3 as template (77). The location of each insertion was verified by PCR using primers 12462 and 12190, which flank the recombination points, and by the ability to use mannose as the sole carbon source. The ΔmanA::cat mutation was subsequently moved into S. enterica strain 14028s by P22-mediated transduction (75) to produce strain MP50.

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