C57BL/6, B6.SJL-PtprcaPepcb/BoyCrCrl (CD45.1+), and C57BL/6-Tg(TcraTcrb)425Cbn/Crl (CD45.2+ WT OT-II) mice were purchased from the Jackson laboratory or Charles River Laboratories. Generation of DGKζ KO, DGKζ floxed (DGKζfl/fl), and DGKα KO mice were described previously (33, 59, 72). CD45.1+CD45.2+ WT OT-II mice were generated by crossing CD45.2+ WT OT-II to CD45.1+ WT mice. CD45.2+ DGKζ KO mice were crossed to CD45.2+ WT OT-II mice to generate CD45.2+ DGKζ KO OT-II mice. C57BL/6-Il4tm1Nnt/J (IL-4 KO) mice were purchased from the Jackson laboratory. B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J (Vav-Cre) and Tg(Cd4-cre)1Cwi/BfluJ (CD4-Cre) mice were purchased from the Jackson laboratory and crossed to DGKζfl/fl mice to generate Vav-Cre DGKζfl/fl and CD4-Cre DGKζfl/fl mice, respectively. B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J (Myh11-Cre) mice were purchased from the Jackson laboratory and crossed to DGKζfl/fl mice to generate Myh11-Cre DGKζfl/fl mice. Pirt-Cre mice were crossed to DGKζfl/fl mice to generate Pirt-Cre DGKζfl/fl mice (73). Sevenmaker (ERKSEM) mice were provided by L. Samuelson from the National Institutes of Health and were originally developed by S. Hedrick from the University of California, San Diego (51). Unless otherwise specified, all mice were 7 to 12 weeks old at the time of use, were housed in pathogen-free conditions, and were treated in strict compliance with the Institutional Animal Care and Use Committee regulations at the University of Pennsylvania.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.