The same technical equipment as for confocal FRET microscopy was used. ImageJ was used to analyze subcellular localization of transcription factors by intensity measurements of the cytosol and nucleus. Three different populations were distinguished with different nucleus/cytosol ratios: inactive (<0.85), homogenous (0.85 to 1.15), and active (>1.15).

All data are presented as means ± SEM for the indicated number of experiments. Statistical significance was determined by unpaired two-sided Student’s t tests for comparison of two groups (using OriginPro 2017). Statistical significance was set to P < 0.05.

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