pLink-SS software was used to initially identify spectra containing disulfide-linked peptides, as previously described (133). Precursor and fragment tolerances were set to 10 ppm, variable modifications of Cys carbamidomethylation, and Ser/Thr phosphorylation. Once a spectrum containing a disulfide-linked peptide was identified, the spectrum was redrawn using a custom R script and manually annotated to include disulfide fragmentation mass shifts of −33.987 atomic mass units (amu) (dehydroalanine) and +31.971 amu (disulfohydryl) at both Cys residues (134).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.