VU0405652 and VU0405645 compounds were formulated as 10% Tween 80 in sterile water at a concentration of 3 mg/ml and administered intraperitoneally to male C57BL6/J mice and dosed at 30 mg/kg. Mouse blood (cardiac puncture) and brains were collected at 15 and 30 min. Animals were euthanized and decapitated, and the brains were removed, thoroughly washed in ice-cold (4°C) phosphate-buffered saline, and immediately frozen on dry ice. Brain samples were processed, and the concentrations of compound were determined by electrospray ionization on an AB SCIEX API 4000 triple-quadrupole instrument that was coupled with Shimadzu LC-10AD pumps and a Leap Technologies CTC PAL auto-sampler. All data were analyzed with AB SCIEX Analyst 1.5.1 software. Compound exposures, in the form of area under the curve, were calculated by the trapezoidal method with PRISM software (GraphPad).

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