Mouse tissues were fixed in 10% neutral buffered formalin (Fisher Scientific, 23-245-685) for 16 hours and dehydrated in a series of ethanol washes up to 100%. Samples were paraffin-embedded and sectioned at 4-μm thickness. For immunohistochemistry, slides were deparaffinized in xylene and rehydrated with a series of ethanol washes. Antigen retrieval was performed using citrate buffer (Electron Microscopy Sciences, 62706-10), and slides were stained using antibodies against GFP that cross-react with YFP (1:200; Abcam), pERK (1:500; CST), and EGFR-XP (1:200; CST). Primary antibody was incubated on slides for 16 hours at 4°C, and biotinylated secondary antibody (Vector Laboratories) was incubated at room temperature for 1 hour. ABC reagent and ImmPACT DAB were prepared as directed (Vector Laboratories, PK-4001 and SK-4105). Slides were analyzed on a Leica DMI6000B inverted microscope. For immunofluorescence, slides were again deparaffinized in xylene and rehydrated with a series of ethanol washes. Antigen retrieval was performed using citrate buffer (Electron Microscopy Sciences, 62706-10), and slides were stained using antibodies against GFP (1:200; Abcam), Ki67 (1:200; Abcam), and EGFR-XP (1:200; CST) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) using Fluoro-Gel II with DAPI (EMS, catalog no. 17985-50). Detection was by Alexa Fluor 488– or Alexa Fluor 594–conjugated secondary antibodies (Molecular Probes). Slides were analyzed on a Leica DMI6000 B inverted microscope.

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