Vδ2+ γδT cells transduced to express CD33-DAP10 or CD33-CD28 CCR were cocultured for 7 days with irradiated MV-411 at a 1:1 effector:target ratio in RPMI 1640 medium supplemented with l-glutamine (2 mM), penicillin (100 IU/ml)/streptomycin (100 μg/ml), 10% FCS (v/v), and IL-2 (100 IU/ml). Absolute numbers of CD3+/Vδ2+/CCR+ and CD3+/Vδ2+/CCR cells in the coculture were determined by flow cytometry using Precision Count Beads (BioLegend), and these values used to derive fold change of each population during the culture period.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.