Vδ2+ γδT cells transduced to express CD33-DAP10 or CD33-CD28 CCR were cocultured for 7 days with irradiated MV-411 at a 1:1 effector:target ratio in RPMI 1640 medium supplemented with l-glutamine (2 mM), penicillin (100 IU/ml)/streptomycin (100 μg/ml), 10% FCS (v/v), and IL-2 (100 IU/ml). Absolute numbers of CD3+/Vδ2+/CCR+ and CD3+/Vδ2+/CCR cells in the coculture were determined by flow cytometry using Precision Count Beads (BioLegend), and these values used to derive fold change of each population during the culture period.

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