β Cell mass was calculated as previously detailed using at least five mice per group (51). Pancreata and isolated islets were stained using the following antibodies: anti-insulin (1:350, #A0564, Dako), anti-Ki67(1:200, #Ab66155-200, Abcam), anti-eIF5AHyp (1:200, IU-44), anti-eIF5ATotal (1:200, #611977, BD Pharmingen), and anti–phospho–PKC-ζ [1:100, SAB4503773, Sigma-Aldrich; 4′,6-diamidino-2-phenylindole (DAPI), Thermo Fisher]. Apoptosis was detected using the ApopTag Red In Situ Apoptosis Detection Kit (Millipore Sigma). Images were acquired using a Zeiss LSM 800 microscope (Carl Zeiss).

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