Cells were filtered through a series of gates to ensure compatibility between compared samples. Singlets were identified using DNA content, and live cells were detected by exclusion of Cell-ID Cisplatin (Fluidigm), which only stains dead cells. T cell subsets were identified by expression of CD3 ± CD4, CD8, or TCRVδ2. CAR expression was detected using anti-human Fc, which stains the stalk portion of the CAR. An example of the gating strategy used is shown in fig. S15A.

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