Cells were filtered through a series of gates to ensure compatibility between compared samples. Singlets were identified using DNA content, and live cells were detected by exclusion of Cell-ID Cisplatin (Fluidigm), which only stains dead cells. T cell subsets were identified by expression of CD3 ± CD4, CD8, or TCRVδ2. CAR expression was detected using anti-human Fc, which stains the stalk portion of the CAR. An example of the gating strategy used is shown in fig. S15A.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.