For specific Vδ2+ γδT cell expansion, PBMCs were isolated as described above. They were cultured in RPMI 1640 medium supplemented with l-glutamine (2 mM, Sigma-Aldrich), penicillin (100 IU/ml)/streptomycin (100 μg/ml) (Sigma-Aldrich), and 10% FCS (v/v) (Gibco, Massachusetts, USA). Vδ2+ γδT cell expansion was stimulated using 5 μM zoledronic acid (Actavis, New Jersey, USA) and IL-2 (100 IU/ml; Aldesleukin, Novartis, Frimley, UK), which was added to PBMC suspension after PBMC isolation (day 1). IL-2 was replenished every 2 to 3 days by removing half of the media from the well and replacing with fresh media containing IL-2 (200 IU/ml).

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