Soluble and insoluble Aβ42 were extracted according to the human brain Aβ42 enzyme-linked immunosorbent assay (Millipore) manufacturer’s instructions. Plates were read at 450 nm on a Spectra Max Plus plate reader (Molecular Devices). For thioflavin S staining, perfused mouse brains were sectioned to 50-μm slices using a vibratome (Leica VT1000 S). Slices were then subjected to the following washes: 70% ethanol (EtOH) for 1 min, 80% EtOH for 1 min, thioflavin S in 80% EtOH for 15 min, 80% EtOH for 1 min, 70% EtOH for 1 min, and then two washes in DI H2O. Coverslips were then mounted on glass slides with Vectashield aqueous mounting media (Vector Labs, catalog no. H1000). Images were captured on a Nikon (Tokyo, Japan) Eclipse Ni upright microscope, using a Nikon Intensilight and Photometrics Coolsnap HQ2 camera to image thioflavin S. Images were captured with Nikon Elements 4.20.02 image capture software using 4× objective [Nikon Plan Fluor 0.13–numerical aperture (NA) objective].

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