MSgg plates solidified with 0.25% agar were prepared a day before the experiment and dried overnight at room temperature. Cells from a single colony isolated on LB plates were grown to mid-logarithmic phase in a 3-ml LB broth culture (4 hours at 37°C with shaking) and diluted to reach equal OD. Then, a 1-μl drop was spotted on the center of the plate. Plates were incubated at 30°C for 10 hours. Images were obtained with a Nikon COOLPIX P510 camera (Nikon). Data were analyzed using AxioVision suite software (Zeiss).

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