An inhomogeneous point pattern analysis (57) was estimated using the Kinhom function from the “spatstat” package for R. It differs from the Ripley’s H analysis described earlier in that it does not assume receptors to be homogeneously distributed in the null condition, for example, if the receptors are concentrated on membrane protrusions. The O-ring (or neighborhood density function) analysis (58), similarly to the G&F analysis, measures the clustering of events at different radii but subtracts the number of events within a smaller radius from the number of events within a larger radius to generate a measure of events within a ring. Unlike the G&F analysis, this method assesses clustering independently of how dispersed the clusters are from each other.

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