In the Ripley’s K function analysis (55), each event is assigned a value, L(r), based on the number of neighboring events within a given search radius (r). This value is adjusted in the Ripley’s H function analysis, L(r) − r, to account for the increasing number of neighbors that would be expected in a random distribution as r increases. Therefore, a random distribution would have no peak on a Ripley’s H function curve. STORM data derived from mAbs labeled with several fluorescent moieties are inherently clustered; however, this analysis and the relative position of the Ripley’s H function peak are informative for comparative study of the different receptors, because they are labeled to a similar extent.

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