The Kcc2 gene-targeting vector was constructed from 129Sv mouse genomic DNA (GenOway, Lyon, France). T906E and T1007E point mutations were inserted into exon 22 and exon 24, respectively. A loxP-flanked neomycin cassette was inserted in intron 22. Thr1007 corresponds to numbers in human and rat, and the mutated residue in mouse is Thr1006. We mention this residue as Thr1007 in this paper to avoid confusion with previous studies performed using rat KCC2 and to be consistent with the mouse study by Moore et al. (34).

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