A single colony was collected into PBS, gently sonicated, and washed in PBS. Next, it was gently centrifuged, resuspended in 10 μl of 1-43 (Thermo Fisher Scientific) membrane stain (20 μg ml−1). The cells were then incubated for 10 min at room temperature, washed twice with PBS, centrifuged, and resuspended in 10 μl of PBS. Then, the cells were placed on a microscope slide and covered with a poly-l-lysine–coated coverslip (Sigma-Aldrich). Cells were visualized and photographed using an Axioplan 2 microscope (Zeiss) equipped with a high-resolution microscopy Axiocam camera, as required. Data were captured using AxioVision suite software (Zeiss).

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