Protein G Dynabeads (1 ml, Invitrogen) were washed and incubated with 200 μg of anti-Lck (1F6) in binding buffer (sodium borate, pH 9) for 1 hour at room temperature. Beads were rinsed and resuspended in 10 ml of binding buffer. Dimethyl pimelimidate (DMP) (50 mg) was added and mixed for 30 min. Beads were rinsed and resuspended in 0.1 M ethanolamine (pH 8) and incubated for 30 min. Unconjugated antibody was released by washing with 0.1 M glycine (pH 3). Beads were washed twice with tris-buffered saline (TBS, pH 7.4). Beads were resuspended in 1 ml of TBS and stored at 4°C.

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