SW48 cells and isogenic counterparts were cultured in RPMI 1640 medium supplemented with fetal bovine serum (FBS) (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). SW403 cells were cultured in L-15 medium with FBS (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). LS-180 cells were cultured in Eagle’s minimum essential medium (EMEM) with FBS (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). LoVo cells were cultured in F12-K medium with FBS (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). HCT116 cells were grown in McCoy’s 5A medium with FBS (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). HCT-15 cells were grown in RPMI 1640 medium supplemented with FBS (10%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). CaCo2 cells were grown in DMEM with FBS (20%), penicillin (100 U/ml), streptomycin (100 μg/ml), and l-glutamine (2 mM). All cells were grown in indicated medium and incubated at 37°C in 5% CO2 unless indicated otherwise in experimental methods. SW48 cells were obtained from Horizon Discovery. SW403, HCT116, HCT-15, CaCo2, SW48, and LoVo were obtained from the American Type Culture Collection.

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