DRGs were extracted from 7-day-old Westar rats, and neurons were dissociated as previously described (8, 19). Cultures were maintained on 10-mm glass coverslips precoated with poly-d-lysine and laminin, and cells were left to grow for 48 hours in Dulbecco’s modified Eagle’s medium supplemented with GlutaMAX I (Invitrogen), 10% fetal bovine serum, penicillin (50 U/ml), and streptomycin (50 μg/ml) in a humidified incubator (5% CO2, 37°C). For transfection with the EYFP-QL or CEPIA constructs, the Lonza Nucleofector I was used, and transfection was done before plating out the cells as described (70).

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