This research was performed in accordance with the Partners Institutional Review Board (protocol no. 2011P000940 and no. 2018P001584). Written informed consent for specimen collection was obtained from all patients included in this study. PIK3CD mutations were initially identified on next-generation sequencing and confirmed by Sanger sequencing using primers PIK3CD_3_4F/R and PIK3CD_23F/R and polymerase chain reaction (PCR) conditions as previously described (19).

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