Depletion of either galectin-3 (ID: s8149) or IL-1β (ID: s223930) was achieved using Silencer Select siRNAs (Life Technologies). A nontargeting scrambled siRNA (ID: 4390843) served as negative control. For knockdown, cells were transfected by a 6-hour incubation with 500 nM siRNA in Lipofectamine 2000 (0.5 μl/100 mm2; Life Technologies) dissolved in Opti-MEM reduced-serum medium (Life Technologies). Levels of galectin-3 or IL-1β were determined by immunoblot and ELISA, respectively, 72 hours after siRNA transfection.

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