Transfections were performed using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions with some modifications. In brief, neuronal cultures older than 12 days in vitro (DIV) were incubated in 500 μl of antibiotic-free medium containing 3 μl of Lipofectamine and 1.5 μg of plasmid DNA for 3 hours. The transfection medium was replaced with original medium, and the neurons were kept for 3 days in the incubator. Experiments were performed on day 3 after transfection.

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