MLS1547 was originally obtained from the National Institutes of Health Molecular Libraries Screening Center Network Library (27) and subsequently synthesized and verified for purity at the University of Kansas Specialized Chemistry Center by K. Frankowski (28). Gαo1-Rluc8, Gβ1, Gγ2-mVenus, Gαs-Rluc8, CAMYEL biosensor, β-arrestin2–mVenus, human D2R-Rluc8, and human D3R-Rluc8 were gifts from J. Javitch and H. Yano at Columbia University. Additional human receptor cDNAs (D4R, β2R, and V2R) were obtained at the cDNA Resource Center (www.cdna.org). Further receptor constructs and mutants were prepared by Bioinnovatise (Rockville, MD). Constructs were prepared in pcDNA3.1 vectors, and inserts were verified by sequencing. LYN–recombinant green fluorescent protein (rGFP) (40) was a gift from M. Bouvier at the University of Montreal. All tissue culture media and supplies were obtained from Thermo Fisher Scientific (Carlsbad, CA). All other compounds and chemicals, unless otherwise noted, were obtained from Sigma-Aldrich (St. Louis, MO).

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