Cells were transfected with siRNAs using Lipofectamine RNAiMAX reagent (Invitrogen) in six-well plates as per the manufacturer’s guidelines for 24 to 72 hours. ON-TARGETplus siRNAs were purchased from Dharmacon: GCN2 (LQ-005314-00-0002), PERK (LQ-004883-00-0002), PKR (LQ-003527-00-0002), PP6c (LQ-009935-00-0002), TSC2 (LQ-003029-00-0002), and nontargeting siRNA (D-001810-01-05). siRNA specific for p53 was purchased from Thermo Fisher Scientific (Silencer Select siRNA, catalog number 4390825).

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