MCF10A cells are a nontransformed human breast epithelial cell line. MCF10A cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 (1:1) supplemented with horse serum (5%), recombinant human epidermal growth factor (20 ng/ml), human insulin (10 μg/ml), hydrocortisone (500 ng/ml), and cholera toxin (10 ng/ml). p53 wild-type MCF10A (p53+/+) cells were obtained from the American Type Culture Collection (ATCC), and p53 knockout MCF10A (p53−/−) cells were obtained from Sigma-Aldrich. A549, HeLa, and BT474 cell lines were obtained from the ATCC and cultured in DMEM supplemented with 10% fetal bovine serum (FBS).

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