Patch-clamp recordings were performed using an Axopatch 200B amplifier interfaced to an ITC-18 input-output board and an iMac G5 computer. Currents were filtered at 2 kHz with a four-pole Bessel filter and sampled at 5 kHz. Stimulation, data acquisition, and analysis were performed using in-house routines developed on the Igor Pro platform. Recordings were performed from CA1 pyramidal neurons, and the holding potential was −70 mV. CA1 pyramidal cells were identified by their location in the pyramidal layer. IPSCs were isolated by the inclusion of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) (10 μM) and D-(−)-2-amino-5-phosphonopentanoic acid (D-APV) (50 μM) in the extracellular solution to block glutamate receptors. mIPSCs were recorded in the presence of TTX (1 μM).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.