The expression vector pcDNA3 was purchased from Invitrogen. An internal control plasmid for transfection efficiency, pRL-TK renilla luciferase (pRL-TK Luc), was purchased from Promega. The synthetic vitellogenin 3xERE-TATA fused to a luciferase reporter gene plasmid, 3xERE Luc, and a full-length human ERα expression plasmid, pcDNA/WT ERα, and pcDNA/SRC-2 expression plasmids have been described previously (51). All the Q375 mutant expression plasmids used were generated by Celplor Inc. (www.celplor.com) using the pENTR system.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.