The expression vector pcDNA3 was purchased from Invitrogen. An internal control plasmid for transfection efficiency, pRL-TK renilla luciferase (pRL-TK Luc), was purchased from Promega. The synthetic vitellogenin 3xERE-TATA fused to a luciferase reporter gene plasmid, 3xERE Luc, and a full-length human ERα expression plasmid, pcDNA/WT ERα, and pcDNA/SRC-2 expression plasmids have been described previously (51). All the Q375 mutant expression plasmids used were generated by Celplor Inc. ( using the pENTR system.

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