At least 5 × 106 cells were used per chromatin immunoprecipitation (ChIP) using the CST SimpleChIP kit (CST #9003) and following the manufacturer’s protocol. Anti-RORγt (clone H-190; Santa Cruz Biotechnology #Sc-28559x) was used at 2 μg per ChIP, and rabbit isotype immunoglobulin G control (provided with CST SimpleChIP kit) was used at the same concentration. The following primers were used to amplify RORγt-bound DNA at Btla gene: −49 site: CACCAGGTCTCCTGATTTGA (forward) and AGGAGTCCCAAGCATGGCA (reverse); −369 site, GGTTGTAAGATACATTACACTG (forward) and TCTAAATTGTTACAGTCTTTAGG (reverse). The following primers were used to amplify RORγt-bound DNA at Il17a gene promoter: CTGAAGAGCTGGGACCTAATG (forward) and GCTCTCTCATGTTCTCTCCTCTC (reverse); CNS-5 site, CCGTTTAGACTTGAAACCCAGTC (forward) and GTACCTATGTGTTAGGAGGCGC (reverse).

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