GFP-GluN2Bwt– and GFP-GluN2B(P553T)–transfected neurons were immunolabeled, and Z-stack images were acquired. The resulting maximum projections were analyzed using “Sholl analysis” ImageJ plugin (72). Dendrites were manually traced with Neuron Studio software (http://research.mssm.edu/cnic/tools-ns.html). Dendritic spines from tertiary neurites were counted and classified into morphological categories (thin, mushroom, and stubby), using Neuron Studio software automatic analysis, followed by manual revision to discard artifacts and/or spines counts redundancy.

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