We collected the electrophysiological data from one neuron per mouse. All data were expressed as means ± SEM. The peak amplitudes of EPSCs were analyzed by Clampfit 8.0 software. The weighted decay time constant (τW) of NMDAR-EPSCs was calculated according to the following formula: τW = (If × τf + Is × τs)/(If + Is) (If and Is, the fast and slow component of NMDAR-EPSCs, respectively; τf and τs, the fast and slow decay time constant, respectively). For Western blots, the scanned digital images were quantified by National Institutes of Health ImageJ software. The relative immunoreactive density of each protein was normalized by the averaged density in control group. For immunocytochemical analysis, the fluorescent intensities were measured with Image-Pro Plus 6 software and normalized by the averaged intensity in control cells. We conducted two group comparisons with Mann-Whitney U test. The data across multiple groups were compared by one-way analysis of variance (ANOVA) followed by post hoc Tukey’s test. The repeated measurement and Bonferroni post hoc tests were performed to compare the data between multiple groups occurring over time. The criterion for statistical significance was P < 0.05.

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