The eighth or ninth leaves of 5-week-old plants were used to prepare abaxial epidermal strips, which were stained with an aqueous solution of toluidine blue (2 mg/ml). Stomatal density was scored as the number of stomata per 1 mm2. ABA inhibition of stomatal opening assays was performed following a previously described protocol (78). The stained epidermal samples were photographed with a Zeiss Axio Scope.A1 with a 20× objective lens. The lengths and widths of the stomata were measured with ImageJ software, and the stomatal aperture index (the width:length ratio) was calculated.

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