Western blot was performed according to standard procedures. Briefly, 20 μg of protein lysates was separated by a 10% SDS–polyacrylamide gel electrophoresis and transferred on a polyvinylidene difluoride (PVDF) membrane and then probed overnight with corresponding primary antibodies (table S1). After incubation with corresponding mouse or rabbit horseradish peroxidase–conjugated secondary antibody, proteins were visualized using ECL Reagent in combination with x-ray films (Fuji) or with the Amersham Imager 600 (GE Healthcare) for densitometric analysis.

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