Primary hepatocytes were transfected 4 to 6 hours after plating with siNonTargeting (60 nM), siSrebp1 (30 nM), or siSrebp2 (30 nM) (Dharmacon) using the Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturers’ recommendations. Medium was replaced the following morning, and cells were used for experiments 36 to 48 hours after infection.

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