For glucose tolerance tests, mice were fasted for 4 hours before the experiment (starting at 8:00 a.m.) and received an intraperitoneal injection of a defined dose of glucose (Carl Roth) as indicated in the figure legends. Glucose concentrations in the blood were measured with Accu-Chek glucometer (Roche) at 0-, 15-, 30-, 60-, 90-, and 120-min time points. Insulin tolerance tests were performed similarly, except that the mice received a defined dose of insulin (Sigma) as indicated in the figure legends.

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