DAG analysis was performed as described in detail before (19). Briefly, lipids were isolated from liver homogenates by the butanol-methanol extraction method. 1,2-Dioctanoyl-sn-glycerol (Enzo Life Sciences) was used as internal standard. Subsequently, lipid extracts were fractionated into lipid classes on a silica matrix column (Phenomenex), and the DAG fraction was analyzed by liquid chromatography–mass spectrometry (LC-MS).

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