Total RNA from WM3682 melanoma cells that were monocultured or cocultured with adipocytes was extracted using TRIzol (Invitrogen). RNA quality was analyzed on Bioanalyzer, and RNA concentrations were determined with NanoDrop. Single-stranded cDNA was generated from the amplified complementary RNA (cRNA) with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix). Samples were hybridized with Clarioum S Human Arrays (Affymetrix) and scanned at the Hebrew University Microarray Core Facility. Array scanning was performed according to the manufacturer’s instructions. Raw data were processed using Transcriptome Analysis Console (Thermo Fisher Scientific).

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