Lipid droplets were stained as previously described (76). Cells were fixed with 4% paraformaldehyde at room temperature for 20 min, incubated with 60% isopropanol at room temperature for 5 min, and stained with 0.3% oil red O (O1391, Sigma-Aldrich) at room temperature for 20 min. After staining, cells were counterstained with Harris hematoxylin solution (HHS16; Sigma-Aldrich) for 45 s. Lipid droplets were photographed using an Olympus BX61 microscope.

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