Histological examination and scoring of palatal fusion

Coronal serial sections (4 μm) were collected and numbered in sequence from the anterior to the posterior of organ culture palatal shelves samples and embryos. The AOF (%) for each section was calculated as the length of mesenchymal confluence divided by total length of adherence at the MES region × 100% (31). The AOF was individually analyzed for anterior, middle, and posterior sections of each sample, and on average, three sections of each sample were analyzed.

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