WM3682 melanoma cells were cocultured or treated with adipocyte-conditioned medium in six-well plates for 3 days. At confluency, the monolayer was wounded by scratching with a plastic pipette tip to create an about 450-μm-diameter strip. Images of wound closure were taken at 0, 19, 24, and 42 hours using an Olympus IX81 microscope and cellSens Dimension software and were analyzed by ImageJ software. Percentage of migration area was calculated by dividing the healed area by the wounded area. Five scratch areas per sample were analyzed.

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