For establishment of stable cell lines, WM3314 and WM1716 cells were transfected with PLKO.1–miR-211 or PLKO.1–scrambled control vectors using jetPEI kit (Polyplus-transfection) according to the manufacturer’s instructions. For luciferase assays, 1 μg of reporter plasmid (TGF-β–responsive reporter, TGFBR1 3′ UTR reporter, mutated TGFBR1 3′ UTR reporter, or MITF reporter) was cotransfected into cells with 40 ng of Renilla control plasmid using jetPEI (Polyplus-transfection). At 48 hours after transfection, luciferase assays were performed using the Dual Luciferase Kit (Promega) according to the manufacturer’s recommendations. For data shown in Fig. 5 (M and N), cells were transfected first with oligonucleotides, and then at 24 hours after transfection, the cells were transfected again with oligonucleotides and luciferase reporter plasmids. Luciferase assays were performed 24 hours after reporter plasmid transfection.

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