For construction of miR-211 and scrambled control, expression vectors, miR-211, and scrambled sequences were cloned into modified PLKO.1 vector containing a cassette coding for luciferase, mCherry, and puromycin as previously described (13). The TGF-β–responsive luciferase construct and TGFBR1 expression plasmid were provided by Y. Henis (Neurobiology Department, The George S. Wise Faculty of Life Sciences, Tel Aviv University). pcDNA3-MITF was described in previous publications (75). The plasmid containing luciferase fused to the TGFBR1 3′ UTR was a gift from E. Galun (The Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem). Site-directed mutagenesis was performed on the miR-211 binding sites in the TGFBR1 3′ UTR using the QuikChange method (Stratagene) according to the supplier’s protocols. Primer sequences for site-directed mutagenesis are listed in table S2.

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