Total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer’s instructions. RNA was quantified by measuring OD260/280. For quantitative reverse transcription PCR (qRT-PCR) analysis of mature miR-211, 20 ng of total RNA was subjected to a TaqMan mRNA assay (Applied Biosystems). Mature miR-211 levels were normalized to levels of RNU48. For mRNA analysis, cDNA prepared using the qScript cDNA Synthesis Kit (Quantabio) was subjected to qRT-PCR using the PerfeCTa SYBR Green FastMix (Quantabio). Data are presented as fold changes relative to control. Primer sequences and manufacturers are listed in table S2.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.