Total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer’s instructions. RNA was quantified by measuring OD260/280. For quantitative reverse transcription PCR (qRT-PCR) analysis of mature miR-211, 20 ng of total RNA was subjected to a TaqMan mRNA assay (Applied Biosystems). Mature miR-211 levels were normalized to levels of RNU48. For mRNA analysis, cDNA prepared using the qScript cDNA Synthesis Kit (Quantabio) was subjected to qRT-PCR using the PerfeCTa SYBR Green FastMix (Quantabio). Data are presented as fold changes relative to control. Primer sequences and manufacturers are listed in table S2.

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