Primary human white subcutaneous pre-adipocytes (HWP; PromoCell) were cultured in pre-adipocyte growth medium (C-27410; PromoCell). At 80 to 90% confluency, differentiation was induced by culturing the cells in differentiation medium (C-27436; PromoCell) for 3 days, followed by culturing in nutrition medium (C-27438; PromoCell) that was renewed every 2 days for 6 to 8 days. NIH3T3-L1 cells were provided by A. Munitz (Department of Microbiology and Clinical Immunology, Sackler School of Medicine, Tel Aviv University). Cells were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 1% penicillin/streptomycin/glutamine (Invitrogen). Differentiation was induced at 80% confluency using 3T3-L1 differentiation kit (DIF001; Sigma-Aldrich) containing insulin (1.5 μg/ml), 500 μM 3-isobutyl-1-methylxanthine (IBMX), and 1 μM dexamethasone for 3 days followed by culturing in complete DMEM supplemented with insulin (1.5 μg/ml) (I0516; Sigma-Aldrich) for an additional 8 days. Medium was renewed every 2 days. Differentiation was validated at day 10 after induction by oil red O staining.

Primary normal human epidermal keratinocytes (NHEK; PromoCell) were cultured in growth medium (C-20011; PromoCell) and were induced to differentiate with high calcium (1.2 mM) for 5 days. Primary normal human dermal fibroblasts (NHDF; PromoCell) were cultured in DMEM. Primary human endothelial cells (HUVEC; PromoCell) were grown in endothelial cell growth medium (C-22011; PromoCell). WM3314, WM3526, WM3682, and WM1716 melanoma cell lines were provided by L. A. Garraway (Department of Medical Oncology and Center for Cancer Genome Discovery, Dana-Farber Cancer Institute, Boston, MA). Cells were cultured in complete DMEM. To establish stable cell lines, cells were selected with puromycin (1 μg/ml) (Sigma-Aldrich). Polymerase chain reaction (PCR) detection kit (Sigma-Aldrich) tested cells for mycoplasma. Characteristics of the melanoma lines are summarized in table S1.

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