The cell spreading assay was performed as described previously (65). Briefly, MEFs or C33A cells at a density of 1 × 105 cells/ml were seeded onto coverslips that were coated with FN and blocked with BSA, as described in the adhesion assay, for 0 to 1 hour at 37°C. Cells were stained with the primary antibody recognizing the focal adhesion protein paxillin, followed by Alexa 488–conjugated secondary antibody. The relative area of spreading was determined by measuring the area circumscribed by paxillin staining and quantitated using Fiji software. Cells of similar nuclear size were used for measurement.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.