RNA was isolated using TRIzol according to the manufacturer’s instructions (Invitrogen). GAPDH was used as internal control gene. Sequences of PCR primers are as follows: β1 integrin (ITGB1), 5′-GAAGGGTTGCCCTCCAGA-3′ (forward) and 5′- GCTTGAGCTTCTCTGCTGTT-3′ (reverse); GAPDH, 5′-GGATTTGGTCGTATTGGG-3′ (forward) and 5′-GGAAGATGGTGATGGGATT-3′ (reverse).

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