Antibodies to paxillin [rabbit polyclonal (poly) Ab; Abcam, ab2264], phalloidin–tetramethyl rhodamine isothiocyanate (Sigma, P1951), tubulin (rat mAb; Millipore, MAB1864), actinin (mouse mAb; Abcam, ab18061), talin (rabbit poly Ab; Abcam, ab71333), β1 integrin [mouse mAb (Abcam, clone 12G10, ab30394) and rat mAb (BD Biosciences, clone 9EG7, 553715)], MB1.2 (rat mAb; Millipore, MAB1997), Rab5 (rabbit mAb; Cell Signaling Technology, 3547), Rab11 (rabbit poly Ab; Thermo Fisher Scientific, 71-5300), Rab7 (rabbit poly Ab; Thermo Fisher Scientific, PA5-22959), IQGAP1 [mouse mAb; BD Biosciences, 610612) and rabbit poly Ab (Abcam, ab86064)], ARF6 (rabbit poly Ab; Cell Signaling Technology, 3546), HA (mouse mAb; Sigma, H9658), E-cadherin (rabbit mAb; Cell Signaling Technology, 3195), GAPDH-peroxidase (mouse mAb; Sigma, G9295), EFA6 (rabbit poly Ab; Atlas Antibodies, HPA059237), EFA6 (rabbit poly Ab; Thermo Fisher Scientific, PA5-31153), and ARNO (goat poly Ab; Sigma, SAB2500109) were purchased, as stated for each above.

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