All constructs were expressed under the control of a cytomegalovirus promotor. HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium with stable glutamine (Biochrom) supplemented with 10% (v/v) fetal bovine serum (FBS Superior; Biochrom), 1 μM all-trans-retinal, and penicillin/streptomycin (100 μg/ml) (Biochrom, Berlin, Germany). Cells were seeded on poly-lysine–coated glass coverslips (1 × 105 cells/ml) and transiently transfected using the FuGENE HD Transfection Reagent (Promega) 28 to 48 hours before measurements.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.