Additional domains for each of the IRAK, R. irregularis, and PknB-related pseudokinase families were initially identified using NCBI’s Batch Web CD-Search Tool against the Conserved Domain Database with an expected value threshold of 0.01 and a maximum of 500 hits per CD-search (133, 134). TM helices were identified using TMHMM 2.0 (135) and the Phobius web server (136). TM helices were annotated as such only if both TMHMM and Phobius predicted the same TM helix region within a 10-residue margin. We verified kinase domain hits using our manually curated protein kinase profiles and removed any overlapping domain predictions.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.