Cytokine production by T cells was measured using the TH1/TH2/TH17 Cytokine Bead Array (560484, BD Biosciences) or by enzyme-linked immunosorbent assay (ELISA) for IFN-γ (eBiosciences) according to the manufacturers’ instructions. The amount of IFN-γ present in total tumor-associated cell cultures was normalized (based on flow cytometry phenotyping) for 50,000 CD4+ T cells.

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